Modular Enzyme- and Light-Based Activation of Cyclopropene-Tetrazine Ligation

ChemBioChem, 20(17), 2222-2226


We describe a modular activation strategy for cyclopropene-tetrazine ligation. This activation strategy uses chemically diverse enzyme- or photolabile protecting groups as cyclopropene reactivity cages. The linkages between the caging groups and cyclopropene are through carbamates, thus permitting the application of diverse cages to allow bioorthogonal reactivity by administering enzymes or light.

Pratik Kumar, PhD
Pratik Kumar, PhD
HHMI Postdoctoral Associate

I am a chemical biologist and I make chemical tools for biology.